Method and apparatus for obtaining aliquot from liquid-based cytological sample

ABSTRACT

Sample vials and methods of processing the sample vials are provided. The sample vial comprises a vial container, a sample collection chamber within the vial container, a vial cap configured to be mated with the vial container to enclose the collection chamber, an aliquot chamber, which may be carried by the vial cap or the vial container, and a valve mechanism for selectively sealing and unsealing the aliquot chamber from the collection chamber. The method may comprise flowing an aliquot of the sample from the collection chamber while the sample within the collection chamber is isolated from an environment exterior to the vial, sealing the aliquot chamber from the collection chamber to isolate the aliquot sample from the remaining sample portion, and transferring at least some of the remaining sample portion from the collection chamber to a microscope slide while the aliquot chamber is sealed from the collection chamber. The method further comprises reserving the slide for cytological screening of the sample, and reserving the aliquot sample for deoxynucleic acid (DNA) testing.

FIELD OF THE INVENTION

The invention pertains to the preparation of cytological samples, andmore specifically, to a method and apparatus for obtaining aliquots fromcytological samples, such as fluid-based Papanicolaou (“Pap”) smears.

BACKGROUND

Cytology is a branch of biology dealing with the study of the formation,structure, and function of cells. As applied in a laboratory setting,cytologists, cytotechnologists, and other medical professionals makemedical diagnoses of a patient's condition based on visual examinationof a sample of the patient's cells. Cytological techniques lendthemselves well to the detection of abnormal cells and disease in thehuman body.

Cytological techniques are widely employed, because collection of cellsamples for analysis is generally less invasive than traditionalsurgical pathological procedures such as biopsies, whereby a tissuesample is excised from the patient using specialized biopsy needleshaving spring loaded translatable stylets, fixed cannulae, and the like.Cell samples may be obtained from the patient by a variety of techniquesincluding, for example, by scraping or swabbing an area, or by using aneedle to aspirate body fluids from the chest cavity, bladder, spinalcanal, or other appropriate area. The cell samples are subsequentlytransferred to a glass slide for viewing under magnification. Fixativeand staining solutions may be applied to the cells on the glass slidefor preserving the sample for archival purposes and for facilitatingexamination.

A typical cytological technique is a Papanicolaou (“Pap”) smear test inwhich cells are scraped from a woman's cervix and analyzed in order todetect the presence of abnormal cells—a precursor to the onset ofcervical cancer. In a conventional Pap smear test, the cells are smearedonto a glass slide at the physician's office, and the labeled slide isthen sent off to a laboratory for analysis under a microscope by acytotechnician. More recently, liquid-based cytological techniques havebeen employed, which offer new ways of collecting, storing, andanalyzing the cervical cells collected during a Pap smear. Instead ofsmearing the cells onto a microscope slide, the physician places thecollected material into a vial filled with a liquid preservation medium,such as PreservCyt® transport medium, and sends the vial capped andlabeled vial to a laboratory for slide preparation. During slidepreparation, any unwanted material, such as bacteria, blood, mucus, orother debris, is filtered from the sample, and the remaining cells areplaced onto a microscope slide as a monolayer specimen for review by acytotechnician. In this manner, the cells are able to be more readilydiscerned and counted to ensure that an adequate number of cells havebeen evaluated. Oftentimes, slide preparation using liquid-basedcytological samples is performed automatically—up to eighty slides atone time. During this automated procedure, air pressure may be used tomanipulated the sample. In particular, suction is used to draw the fluidonto a cylindrical filter, and a blast of air is to transfer the cellsfrom the filter onto the slide to create the specimen.

Whether a conventional or a fluid-based Pap smear is performed, thespecimen will be classified as either normal or abnormal based on themicroscopic analysis of the slide. An abnormal sample can be classifiedinto one of the major categories defined by The Bethesda System forReporting Cervical/Vaginal Cytologic Diagnosis, which categories includeLow-Grade Squamous Intraepithelial Lesions (LSIL), High-Grade SquamousIntraepithelial Lesions (HSIL), Squamous Cell Carcinoma, Adenocarcinoma,Atypical Glandular cells of Undetermined Significance (AGUS),Adenocarcinoma in situ (AIS), and Atypical Squamous Cell (ASC), whichcan be further sub-divided into Atypical Squamous Cell, cannot excludeHSIL (ASC-H) and Atypical Squamous Cell of Undetermined Significance(ASC-US).

If a specimen is not classified as abnormal after analysis, the Papsmear is considered normal and the patient is returned to a routine papscreen schedule (typically, once a year). With the exception of ASC-US,if a specimen is classified into any one of these abnormal categories,the patient must undergo an inconvenient and relatively painfulcolposcopy and biopsy to further diagnose the presence of cancer orprecancer (dysplasia). Until recently, if a specimen was classified asASC-US, the patient had to undergo repeated Pap smears every few monthsin hopes of determining the nature of the abnormal cells, i.e., whetherthey might develop into cancerous lesions or clear up on their own. Insome cases, a colposcopy and biopsy was performed.

Since 2000, a specific Human Papilloma Virus (HPV) deoxynucleic acid(DNA) test, referred to as the Hybrid Capture II HPV DNA assay,manufactured by Digene Corporation, has been used to determine whetherpatients, whose Pap smears have been classified as ASC-US, have HPV, thehigh-risk serotypes (16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, and68) of which have been recognized as a necessary factor for thedevelopment of cervical cancer (over ninety percent of women withcervical cancer have high-risk HPV).

Based on the strong correlation between HPV and cervical cancer, it hasbeen recommended that HPV DNA testing be used as a triage test forpatients whose Pap smear results are classified as ASC-US. That is,patients who have ASC-US Pap smear results and negative HPV test resultscan be reassured that their short term risk of developing cervicalcancer is very low, and that they could safely return to a routine Papsmear schedule. In contrast, women who have ASC-US Pap smear results andpositive results for high-risk HPV should undergo the colposcopy/biopsyprocess in the same manner as if the Pap smear would have originallybeen classified as LSIL, HSIL, or any of the other abnormal categories.A recent study has shown that performing an HPV DNA test wouldultimately spare forty to sixty percent of patients from undergoing acolposcopic/biopsy examination.

In the case where a conventional Pap smear has been performed, thepatient will typically have to make another visit to the physician toobtain another sample for HPV DNA testing. In the case where aliquid-based Pap smear has been performed, however, the same sample usedto perform the Pap smear analysis can be conveniently used to perform a“reflexive” HPV DNA test, thereby obviating the need for a repeat clinicvisit and Pap smear. In this case, if a slide is positive for ASC-US, analiquot (e.g., 4 ml) of the fluid sample is removed from the stored vialand sent to a molecular diagnostic laboratory for HPV DNA testing.

Theoretically, an HPV DNA test can be performed at any time during thePap smear process. However, because it is currently not commerciallypractical to perform the HPV DNA test in conjunction with every Papsmear, the HPV DNA test is typically performed in response to a ASC-USPap smear result. Thus, in the case of liquid-based Pap smears, thealiquot required for HPV DNA testing would have to be taken from thesample after being processed for slide preparation, which maypotentially lead to molecular contamination issues, and in the case ofautomated Pap smear processing, aerosol cross-contamination resultingfrom the use of air pressure to manipulate the sample or liquidcontamination resulting from the transfer of filtered solution found inthe plumbing of the automated processor. Molecular contamination is nota trivial issue because the transfer of just one nucleic molecule canproduce a false-positive for HPV.

Significantly, laboratories that perform HPV DNA tests are weary ofmolecular contamination—a well-known problem in molecular diagnosticlaboratories, which typically employ special engineering and designfeatures, laboratory practices, and monitoring activities to minimizemolecular contamination in all of their activities. Because automatedsystems for making Pap smear slides are designed to safely andeffectively handle cells—not nucleic acids, the sterilization protocolsfollowed by technicians when operating these systems do not satisfy thestrict molecular contamination control safeguards and proceduresrequired by molecular diagnostic laboratories. Thus, due to the risk ofcross-contamination, molecular diagnostic laboratories may not acceptaliquots that have been taken from an already processed liquid-based Papsmear for fear of unnecessarily generating false HPV positives.

Besides being used for HPV DNA testing, aliquots from liquid-based Papsmear samples can also be used in DNA testing for other sexuallytransmitted diseases, such as Chlamydia trachomatis and Neisseriagonorrhoeae. However, false positives are a special problem when testingfor Chlamydia trachomatis and Neisseria gonorrhoeae, because they couldhave enormous family and social repercussions. Thus, moleculardiagnostic laboratories are even more reluctant to accept aliquots fromalready processed liquid-based Pap smear samples. Because testing forother sexually transmitted diseases need not be used to triage ASC-USspecimens, and are intended to be performed in parallel to the Pap smeartests at the request of the physician, aliquots may be taken from thePap smear samples prior to processing, e.g., by manually pipetting thealiquot from the vial, thereby minimizing the risk ofcross-contamination. However, this step may still not satisfy the strictcontamination prevention requirements imposed by molecular diagnosticlaboratories.

Besides contamination issues, the pipetting of an aliquot from afluid-based Pap smear sample, whether done before or after the sample isprocessed, and whether done for HPV testing or testing of any othersexually transmitted disease, increases cost in the form of manual labor(which involves not only pipetting the aliquot into an extra vial, butalso labeling the vial), material costs for the pipettes and extravials, and storage space, which when multiplied by the thousands ofsamples processed, becomes significant.

There thus is a need to provide an improved method and apparatus forobtaining an aliquot from a fluid-based cytological sample, such as aPap smear sample.

SUMMARY OF THE INVENTION

In accordance with a first aspect of the present inventions, a method ofprocessing a vial having a collection chamber containing a fluid-basedcervical-vaginal sample, and an aliquot chamber, is provided. The methodcomprises flowing an aliquot of the sample from the collection chamberinto the aliquot chamber while the sample within the collection chamberis isolated from the exterior environment. One method comprisesunsealing the aliquot chamber from the collection chamber, in whichcase, the sample aliquot is flowed from the collection chamber into theunsealed aliquot chamber.

The method further comprises sealing the aliquot chamber from thecollection chamber to isolate the aliquot sample from the remainingsample portion in the collection chamber, and transferring at least someof the remaining sample portion from the collection chamber to amicroscope slide while the aliquot chamber is sealed from the collectionchamber. The vial may have a vial container carrying the collectionchamber and a vial cap (which may optionally carry the aliquot chamber)mated with the vial container, in which case, the method may furthercomprise unmating the vial cap from the vial container to expose theremaining sample portion within the collection chamber. Removal of thevial cap may expose the remaining sample portion within the collectionchamber to a HPV contaminant at a molecular level. For example, if someof the remaining sample portion is automatically transferred from thecollection chamber to the slide, the remaining sample portion may beexposed to the HPV contaminant when automatically transferred to theslide. However, because the aliquot sample is isolated within thealiquot chamber from the collection chamber, the aliquot sample will notbe exposed to any HPV contaminant that enters the collection chamber.

The method further comprises reserving the slide for cytologicalscreening of the sample for precursors to cervical cancer, and reservingthe aliquot sample for deoxynucleic acid (DNA) testing to determine thepresence of high-risk Human Papilloma Virus (HPV) in the sample. Themethod may optionally comprise examining the slide to cytologicallyscreen the sample for the cervical cancer precursors, and DNA testingthe aliquot sample to determine the presence of high-risk HPV in thesample. In one method, the DNA testing is only performed in response toan abnormal result of the cytological screening, e.g., an AtypicalSquamous Cell of Undetermined Significance (ASC-US) result.

In accordance with a second aspect of the present inventions, anothermethod of processing a vial having a collection chamber containing afluid-based sample (e.g. a cervical-vaginal sample), and an aliquotchamber, is provided. The method comprises unsealing the aliquot chamberfrom the collection chamber, flowing an aliquot of the sample from thecollection chamber into the aliquot chamber, and sealing the aliquotchamber from the collection chamber in a similar manner described above.The method further comprises removing at least some of the remainingsample portion from the collection chamber while the aliquot chamber issealed from the collection chamber. Optionally, at least some of theremaining sample portion is transferred to a microscope slide.

Significantly, if the remaining sample portion is exposed to anycross-contaminants from another sample during removal of some of theremaining sample portion from the collection chamber, the isolatedaliquot sample will not be exposed to any cross-contaminant that entersthe collection chamber. Cytological examination and DNA testing can thenbe performed on the respective removed remaining sample portion andaliquot sample in the manner discussed above.

In accordance with a third aspect of the present inventions, a samplevial is provided. The sample vial comprises a vial container and asample collection chamber within the vial container. The sample vial maycontain a fluid-based sample (e.g., a cervical-vaginal sample) withinthe collection chamber. The sample vial further comprises an aliquotchamber, and a vial cap configured to be mated with the vial containerto enclose the collection chamber. The vial may optionally comprise asealed port configured to selectively provide direct access to thealiquot chamber. The aliquot chamber may abut the collection chamber,but in alternative embodiments, the aliquot chamber may be coupled tothe collection chamber via an intermediate passageway. The vial furthercomprises a valve mechanism for selectively sealing and unsealing thealiquot chamber from the collection chamber. Although the presentinventions should not be so limited in their broadest aspects, sealingand unsealing the aliquot chamber from the collection chamber allows analiquot of a sample to be flowed from the collection chamber into thealiquot chamber and isolated from the remaining sample portion in thecollection chamber. In one embodiment, the vial cap carries the aliquotchamber and the valve mechanism. In another embodiment, the vialcontainer carries the aliquot chamber and the valve mechanism.

In one embodiment, the valve mechanism includes a valve configured to beselectively located within the aliquot chamber to prevent fluidcommunication between the aliquot chamber and the collection chamber,and displaced from the aliquot chamber to allow fluid communicationbetween the aliquot chamber and the collection chamber. In anotherembodiment, the valve mechanism includes a valve configured to beselectively located at an interface between the aliquot chamber and thecollection chamber to prevent fluid communication between the aliquotchamber and the collection chamber, and displaced from the interface toallow fluid communication between the aliquot chamber and the collectionchamber.

In either case, the valve mechanism may include an actuator that can beexternally manipulated to selectively seal and unseal the aliquotchamber from the collection chamber with the valve. As examples, theactuator may be configured to be rotationally translated to operablymove the valve relative to the aliquot chamber (e.g., the actuator canbe rotated one way to seal the aliquot chamber from the collectionchamber, and rotated the other way to unseal the aliquot chamber fromthe collection chamber) or axially translated to operably move the valverelative to the aliquot chamber (e.g., the actuator can be pulled upwardto seal the aliquot chamber from the collection chamber, and pusheddownward to unseal the aliquot chamber from the collection chamber). Inthe latter case, a spring may be coupled to the actuator to urge thevalve to seal the aliquot chamber from the collection chamber to obviatethe need to pull the actuator upward.

Other and further aspects and features of the invention will be evidentfrom reading the following detailed description of the preferredembodiments, which are intended to illustrate, not limit, theinventions.

BRIEF DESCRIPTION OF THE DRAWINGS

The drawings illustrate the design and utility of preferred embodimentsof the present invention, in which similar elements are referred to bycommon reference numerals. In order to better appreciate how theabove-recited and other advantages and objects of the present inventionsare obtained, a more particular description of the present inventionsbriefly described above will be rendered by reference to specificembodiments thereof, which are illustrated in the accompanying drawings.Understanding that these drawings depict only typical embodiments of theinvention and are not therefore to be considered limiting of its scope,the invention will be described and explained with additionalspecificity and detail through the use of the accompanying drawings inwhich:

FIG. 1 is a perspective view of one embodiment of a sample vialconstructed in accordance with the present invention;

FIG. 2 is a cross-sectional exploded view of the sample vial of FIG. 1,particularly showing a valve mechanism in an open position;

FIG. 3 is a cross-sectional exploded view of the sample vial of FIG. 1,particularly showing the valve mechanism is the closed position;

FIG. 4 is a close-up cross-sectional view of a vial cap of the samplevial of FIG. 2;

FIG. 5 is a close-up cross-sectional view of an alternative vial capthat can be used with the sample vial of FIG. 1, particularly showing avalve mechanism in an open position;

FIG. 6 is a close-up cross-sectional view of vial cap of FIG. 5,particularly showing the valve mechanism in a closed position;

FIG. 7 is a close-up cross-sectional view of another alternative vialcap that can be used with the sample vial of FIG. 1, particularlyshowing a valve mechanism in an open position;

FIG. 8 is a close-up cross-sectional view of vial cap of FIG. 7,particularly showing the valve mechanism in a closed position;

FIG. 9 is a cross-sectional exploded view of another embodiment of asample vial constructed in accordance with the present inventions,particularly showing a valve mechanism in an open position;

FIG. 10 is a cross-sectional exploded view of the sample vial of FIG. 9,particularly showing the valve mechanism is the closed position;

FIG. 11 is a close-up cross-sectional view of a vial cap of the samplevial of FIG. 10; and

FIG. 12 is a flow diagram of a method for processing the foregoingsample vials.

DESCRIPTION OF ILLUSTRATED EMBODIMENTS

Referring to FIG. 1, a sample vial 10 constructed in accordance with oneembodiment of the present invention will be described. The vial 10 maybe used to contain a fluid-based sample, such as a cervical-vaginalsample collected from a patient at a physician's office. The fluid-basedsample typically comprises cytological material suspended in an aqueouspreservative fluid.

To this end, the vial 10 comprises a hollow vial container 12 and a vialcap 14 that can be placed onto the vial container 12 to enclose a samplecontained within the vial container 12. As depicted, the vial container12 and vial cap 14 are generally cylindrical in shape. The selected sizeof the vial container 12 and vial cap 14 may vary, but preferably islarge enough to contain the minimum amount of sample necessary toperform the intended diagnostic test. In the illustrated embodiment, thevial container 12 is capable of containing at least 20 ml of fluid,which is the minimum amount of sample required by the Food and DrugAdministration (FDA) for automated transfer onto a microscope slideusing Cytyc's ThinPrep® 2000 or Thinprep® 3000 slide preparationsystems. For example, the vial container 12 may have an outer diameterof approximately 1 and 5/16 inches and an axial length of approximately2 and ¾ inches, and the vial cap 14 may have an outer diameter ofapproximately 1 and 9/16 inches and an axial length approximately 7/16of an inch.

The vial container 12 is composed of a translucent or transparentmaterial to allow a user to determine the fluid level inside of the vial10. A suitable material is a plastic, such as polypropylene homopolymer,available under the trade designation AMOCO 4018. The vial cap 14 may bereleasably mated with the vial container 12 using a standard threadedengagement (not shown), and may be composed of a plastic material, suchas polypropylene random copolymer, available under the trade designationAMOCO 8949. The materials of which the vial container 12 and vial cap 14are composed may be injection molded to rapidly and inexpensivelyproduced the container 12 and cap 14, although other suitablemanufacturing processes may be utilized depending on the particularmaterials selected.

A seal (not shown) may be disposed between the vial container 12 and cap14 to form a fluid-tight seal when sufficient torque is applied to thecap 14 relative to the container 12. Sealing is important to preventboth leakage and evaporation of the preservative solution in the vialcontainer 12, as well as to prevent the sample from being exposed toexternal contaminants. The seal may be composed of any material ormaterials capable of withstanding attack by the preservation solution inthe vial container 12, which typically includes an alcohol solution,such as methanol in a buffer. Due to the low viscosity and high vaporpressure of the preservative solution, as well as the very low densityand high permeability of the vapor phase thereof, a high integrity,reliable, seal composition is desired. Further, because the vial 10 maybe stored for a year or more prior to use, and be subject to temperatureextremes during transport and storage, the seal should be capable ofretaining its sealing characteristics and structural integrity forextended periods of time without excessive loss of fluid due toevaporation. The seal material also should not degrade and contaminatethe sample. In one embodiment that meets these requirements, the seal iscomposed of a multicomposite material, including a sufficiently thick,dense, resilient layer disposed on a vapor barrier. The resilient layermay be oriented toward the sample to provide an effective seal. The sealmay include a synthetic olefin rubber or an elastomeric alloyco-extruded on a thin vapor barrier, such as that available fromTri-Seal, Inc., located in Blauvelt, N.Y., and sold under the trade nameTRI SEAL SOR-117.

The vial container 12 includes a fluid level indicia 16 by which a usermay determine a proper amount of preservation fluid to fill the vial 10or that the vial 10 is filled properly prior to addition of thecytological material. The fluid level indicia 16 may be a frostedannular band of a predetermined axial length disposed about acircumference of the vial container 12 at a predetermined axial locationto indicate the acceptable fill range of the vial 10, so that a properslide sample can be prepared from the sample by an automated specimenpreparation system, such as Cytyc's ThinPrep® 2000 or ThinPrep® 3000slide preparation systems. Alternatively, the fluid level indicia 16 maybe a single fill line or an upper fill line and a lower fill line, inwhich case, the upper fill line indicates a maximum level to which thevial container 12 should be filled and the lower fill line indicates aminimum amount of fluid necessary to prepare a specimen from the sample.

The vial container 12 also includes sample indicia 18, which can be usedto identify a patient to whom the sample corresponds, as well as a slideprepared from the sample contained in the sample vial 10. The sampleindicia 18 may be machine-readable, such as a bar code, which can beready by an automated cytological specimen preparation system, such asCytyc's ThinPrep® 2000 or ThinPrep® 3000 slide preparation systems.

In an optional embodiment, the vial container 12 and vial cap 14 may bespecially configured for automated manipulation. For example, the vialcontainer 12 may have laterally protruding anti-rotation lugs (notshown), and the vial cap 14 may have a torque pattern of ribs (notshown), thereby allowing the cap 14 to be screwed on and screwed off ofthe vial container 12 using automated machinery. Additional detailsregarding these features are disclosed in U.S. patent application Ser.No. 09/156,952, entitled “Sample Vial for Use in Preparing CytologicalSpecimen,” which is fully and expressly incorporated herein byreference.

Referring further to FIGS. 2 and 3, the vial 10 includes a feature thatallows an aliquot sample to be taken and isolated from the samplecontained within the vial container 12. In particular, the vial 10comprises a collection chamber 20 formed within the vial container 12for collection of the sample, an aliquot chamber 22 for containing thealiquot sample, and a valve mechanism 24 for selectively sealing andunsealing the aliquot chamber 22 from the collection chamber 20, so thealiquot sample can be transferred from the collection chamber 20 intothe aliquot chamber 22 where it can be isolated from the remainingportion of the sample within the collection chamber 20. In theillustrated embodiment, the aliquot chamber 22 abuts the collectionchamber 20, although alternatively, aliquot chamber 22 may communicatewith the collection chamber 20 via, e.g., a passageway.

In the embodiment illustrated in FIGS. 2 and 3, the aliquot chamber 22and valve mechanism 24 are carried by the vial cap 14. In particular,the vial cap 14 includes an outer annular flange 26, an inner annularflange 28, and an annular space 30 between the flanges 26, 28. The vialcontainer 12 includes a lip 32 that is sized to fit within the annularspace 30 of the vial cap 14 in a snug manner, so that the inner surface34 of the outer annular flange 26 bears against the outer surface 36 ofthe vial container 12, and the outer surface 38 of the inner annularflange 28 bears against the inner surface 40 of the vial container 12,as best illustrated in FIG. 4. In the illustrated embodiment, the innersurface 34 of the outer annular flange 26 and the outer surface 36 ofthe vial container 12 include threads (not shown), so that the vial cap14 can be firmly screwed on the vial container 12. As can seen, theinner annular flange 28 of the vial cap 14 defines the aliquot chamber22 therein, which absent the valve mechanism 24, would normally be influid communication with the collection chamber 20 when the vial cap 14is mated with the vial container 12.

The valve mechanism 24 includes a valve 42 that is configured todirectly interface with the aliquot chamber 22 to allow or prevent fluidcommunication with the collection chamber 20. In the embodimentillustrated in FIGS. 2 and 3, the valve 42 is configured to beselectively displaced from the aliquot chamber to allow fluidcommunication between the aliquot chamber 22 and the collection chamber20 (FIG. 2), and located within the aliquot chamber 22 to prevent fluidcommunication between the aliquot chamber 22 and the collection chamber20 (FIG. 3).

As best shown in FIG. 4, when preventing fluid communication between therespective chambers 20, 22, the valve 42 sealingly bears against theinner surface 44 of the inner annular flange 28. To this end, the valve42 includes an annular flange 46 that has a diameter slightly smallerthan the diameter of the aliquot chamber 22 and an O-ring seal 48 seatedwithin an annular recess 50 formed around the circumferential edge ofthe annular flange 46, so that the total diameter of the valve 42 isslightly greater than the diameter of the aliquot chamber 22 in order tofacilitate the sealing arrangement.

The valve mechanism 24 further includes an actuator 52, which includes ashaft 54 coupled to the valve 42 and a boss 56 coupled to the end of theshaft 54 and extending up through an upper bore 58 formed in the vialcap 14. As such, the boss 56 can be externally manipulated toselectively displace the valve 42 from the aliquot chamber 22 by axiallymoving the actuator shaft 54 downward, and locate the valve 42 withinthe aliquot chamber 22 by axially moving the actuator shaft 54 upward.The annular flange 46 of the valve 42, the shaft 54, and the boss 56 maybe conveniently formed from the same material as a unibody design, e.g.,in an injection molding process using a plastic material, such aspolypropylene or Acrylonitrile Butadiene Styrene (ABS).

As best shown in FIG. 4, in order to prevent fluid communication betweenthe aliquot chamber 22 and the external environment that may otherwiseoccur through the upper bore 58, the valve mechanism 24 includes anO-ring seal 60 seated within an annular recess 62 formed around thecircumference of the shaft 54. The O-ring seal 60 axially moves withinan enlarged lower bore 64 between the upper bore 58 and the aliquotchamber 22. In the illustrated embodiment, the lower bore 64 is definedby an innermost annular flange 66 formed within the vial cap 14, so thatthe O-ring seal 60 bears against an inner surface 68 of the innermostannular flange 66. Thus, the O-ring seal 60 axially moves within thelower bore 64 in a sealing arrangement to allow the actuator shaft 54,and thus, the valve 42, to move up or down relative to aliquot chamber22, while preventing fluid communication between the aliquot chamber 22and the external environment through the upper bore 58.

In the embodiment illustrated in FIGS. 2 and 3, axial movement of theactuator shaft 54 is accomplished by rotationally translating the boss56 (shown by arrow), and thus, the shaft 54, to operably move the valve42 relative to the aliquot chamber 22. To this end, as best shown inFIG. 4, a threaded arrangement 70 is provided between the actuator shaft54 and the upper bore 58. Thus, clockwise rotation of the boss 56 causesthe shaft 54, and thus the valve 42, to move axially downward, andcounterclockwise rotation of the boss 56 causes the shaft 54, and thusthe valve 42, to move axially upward. As can be appreciated, the boss56, which is larger than the shaft 54, allows the user to more easilyand ergonomically rotate the shaft 54 against any frictional resistancecaused by the interaction of the valve 42 and aliquot chamber 22. Theboss 56 can be further provided with a knurled surface (not shown) tofacilitate gripping by the user. Alternatively, rather than using a bossor any other element that protrudes from the top of the vial cap 14, aslot or other suitable pattern can be formed at the end of the shaft 54to allow a tool, such as a screw driver, to be mated with the shaft 54for subsequent rotation thereof.

To allow user access to the aliquot sample, the vial cap 14 includes anaccess port 72 adjacent the aliquot chamber 22 and a sealing mechanismin the form of septum 74 seated within the access port 72 to seal it,thereby preventing fluid communication from the aliquot chamber 22through the access port 72 until the user is ready to remove the aliquotsample from the aliquot chamber 22 for examination. User access to thealiquot sample can be accomplished, e.g., by puncturing the septum 74with a syringe (not shown) and drawing the aliquot sample from thechamber 22 into the syringe. Alternatively, a seal may be bonded on topsurface of the vial cap 14 above the access port 72 or a screw-on plugor cap can be used to seal the access port 72.

Referring now to FIGS. 5 and 6, an alternative embodiment of a vial cap84 will be described. The vial cap 84 is identical to the vial cap 14illustrated in FIGS. 2 and 3, which the exception that it comprises avalve mechanism 86, wherein the actuator shaft 54 is configured to beonly axially translated, i.e., without rotational translation. In thiscase, there is no threaded arrangement between the shaft 54 and theupper bore 58. Rather, the actuator shaft 54 is slidably engaged withthe bore 58 in the axial direction, so that a user may simply push orpull the shaft 54 to axially move the valve 42 relative to the aliquotchamber 22. The valve mechanism 86 comprises a spring 88 disposedbetween an annular flange 90 inwardly extending from the innermostannular flange 66 at the bottom of the enlarged lower bore 64 and anannular flange 92 outwardly extending from the actuator shaft 54 justbelow the O-ring seal 60.

In this manner, the spring 88 urges the actuator shaft 54 axiallyupward, and thus, the valve 42 into the aliquot chamber 22. As such,absence any external force, fluid communication between the aliquotchamber 22 and the collection chamber 20 (shown in FIGS. 2 and 3) isprevented. However, when a user pushes axially downward on the boss 56,and thus the actuator shaft 54, against the urging force of the spring88, the valve 42 is displaced from the aliquot chamber 22, therebyallowing fluid communication between the aliquot chamber 22 and thecollection chamber 20 (FIG. 5). When the user releases the boss 56, andthus the shaft 54, the urging force of the spring 88 will cause theactuator shaft 54 to axially move upward, thereby moving the valve 42back into the aliquot chamber 22 to prevent fluid communication with thecollection chamber 20 (FIG. 6). In an alternative embodiment, the boss56 is eliminated, and the user need only push down or release the top ofthe actuator shaft 54. The end of the actuator shaft 54 may be recessedwithin the vial cap 84, in which case, the user may push down on theactuator shaft 54 using a simple tool.

In the previously illustrated embodiments, the valve mechanismsselectively prevent fluid communication between the aliquot chamber 22and collection chamber 20 by placing the valve within the aliquotchamber 22. In other embodiments, the valve mechanism may have a valvethat directly interfaces with the aliquot chamber 22 in other manners toprevent such fluid communication.

For example, referring to FIGS. 7 and 8, another alternative embodimentof the vial cap 94 will be described. The vial cap 94 is identical tothe vial cap 14 illustrated in FIGS. 2 and 3, with the exception that itcomprises a valve mechanism 96 that seals the aliquot chamber 22 at theinterface with the collection chamber 20. In particular, the valvemechanism 96 comprises a valve 98 configured to be selectively displacedfrom a lower-most edge 100 of the inner annular flange 28 to allow fluidcommunication between the aliquot chamber 22 and the collection chamber20 (FIG. 7), and placed against the lower-most edge 100 of inner annularflange 28 to prevent fluid communication between aliquot chamber 22 andcollection chamber 20 (FIG. 8).

When preventing fluid communication between the respective chambers, thevalve 98 sealingly bears against the lower-most edge 100 of the innerannular flange 28. To this end, the valve 98 includes an annular flange102 that has a diameter greater than the diameter of the aliquot chamber22 and an O-ring seal 104 seated within an annular recess 106 within theupper surface of the flange 102, so that the O-ring seal 104 can contactthe lower-most edge 100 of the inner annular flange 28 in order tofacilitate the sealing arrangement.

In the same manner described above with respect to FIGS. 2 and 3, axialmovement of the actuator shaft 54 is accomplished by rotationallytranslating the boss 56 (shown by arrow), and thus, the shaft 54, tooperably move the valve 98 relative to the aliquot chamber 22. That is,clockwise rotation of the boss 56 causes the shaft 54, and thus thevalve 98, to move axially downward, and counterclockwise rotation of theboss 56 causes the shaft 54, and thus the valve 98, to move axiallyupward. Alternatively, in the same manner described above with respectto FIGS. 5 and 6, the valve mechanism may be configured, such that theshaft 54 need only be axially translated, i.e., without rotationaltranslation.

It should be appreciated that the incorporation of the aliquot chamberand valve assembly into the vial cap, as illustrated in FIGS. 1-8,allows the vial 10 to be stored upright in a standard manner withouthindrance by any portion of the valve mechanism protruding from the vialand without risk that the remaining portion of the sample contained inthe collection chamber 20 will leak into the aliquot chamber 22 with thevial 10 is stored upright. In addition, incorporation of the aliquotchamber into the vial cap provides the option of separating the vial capcontaining the aliquot sample from the vial body, transferring theseparated vial cap with the contained sample aliquot to a differentlocation for molecular testing, and recapping and leaving the vial bodyat the cytological laboratory for producing a slide specimen or for anyother reason. However, in this case where the aliquot chamber isincorporated into the vial cap, the vial 10 will need to be turnedupside down to flow the sample aliquot from the collection chamber 20into the aliquot chamber 22. In the case where an automated specimenpreparation process is to be used to transfer the aliquot sample intothe aliquot chamber 22, this would require an additional step offlipping the vial upside down. Also, because the aliquot sample will besubsequently tested, it is prudent that separate sample indicia (notshown) be placed on the vial cap 14 in addition to the vial container 12to eliminate or minimize the possibility that vial caps and vialcontainers will be incorrectly mated together.

As illustrated in FIGS. 9 and 10, an embodiment of a sample vial 110,wherein the aliquot chamber and valve mechanism are integrated into thevial container, will now be described. In this case, the vial 110 neednot be turned upside down to flow the sample aliquot from the collectionchamber into the aliquot chamber, and because the aliquot chamber is notincorporated into the vial cap, separate sample indicia is not requiredfor the vial cap. In particular, the vial 110 comprises a hollow vialcontainer 112 and a vial cap 114 that can be placed onto the vialcontainer 112 to enclose a sample contained within the vial container112. The vial container 112 and vial cap 114 are identical to the vialcontainer 12 and vial cap 14 illustrated in FIGS. 1-3 in all respects,with the following exceptions.

Because the vial cap 114 does not carry an aliquot chamber and valvemechanism, the vial cap 114 may be a standard vial with includes asingle annular flange 126 for mating with the top of the vial container112. Like the vial 110 illustrated in FIGS. 2 and 3, the vial 110comprises a collection chamber 120 formed within the vial container 112for collection of the sample, an aliquot chamber 122 for containing thealiquot sample, and a valve mechanism 124 for selectively sealing andunsealing the aliquot chamber 122 from the collection chamber 120, sothe aliquot sample can be transferred from the collection chamber 120into the aliquot chamber 122 where it can be isolated from the remainingportion of the sample within the collection chamber 120. However, unlikethe vial illustrated in FIGS. 2 and 3, the aliquot chamber 122 and valvemechanism 124 are carried by the vial container 112.

In particular, an annular flange 128 is formed at the bottom of the vialcontainer 112 to define the aliquot chamber 122, which absent the valvemechanism 124, would normally be in fluid communication with thecollection chamber 120. The valve mechanism 124 includes a valve 142that is configured to directly interface with the aliquot chamber 122 toallow or prevent fluid communication with the collection chamber 120. Inthe embodiment illustrated in FIGS. 9 and 10, the valve 142 isconfigured to be selectively displaced from the aliquot chamber to allowfluid communication between the aliquot chamber 122 and the collectionchamber 120 (FIG. 9), and located within the aliquot chamber 122 toprevent fluid communication between the aliquot chamber 122 and thecollection chamber 120 (FIG. 10).

The valve 142 interfaces with the annular flange 128 in the same manneras the valve 42 interfaces with the inner annular flange 28 of the vialcap 14 illustrated in FIGS. 2 and 3. That is, as best shown in FIG. 11,when preventing fluid communication between the respective chambers, thevalve 142 sealingly bears against the inner surface 144 of the annularflange 128. The valve 142 includes an annular flange 146 that has adiameter slightly smaller than the diameter of the aliquot chamber 122and an O-ring seal 148 seated within an annular recess 150 formed aroundthe circumferential edge of the flange 146, so that the total diameterof the valve 142 is slightly greater than the diameter of the aliquotchamber 122 in order to facilitate the sealing arrangement.

Notably, because the annular flange 128 is set off from the outer wallof the vial container 112, the risk of interfering with the sealingrelationship between the valve 142 and the inner surface 144 of theannular flange 128 is minimized. That is, if an inner annular flange isnot used, and instead, the valve 142 sealingly interfaces with the innersurface of the outer wall of the vial container, the act of simplygrasping the vial container may warp the outer wall, thereby breakingthe sealing relationship between the valve 142 and outer wall.Alternatively, if the outer wall of the vial container 112 is sturdyenough, the valve 142 could be made to interface with the inner surfaceof the outer wall without risk of interfering with the sealingrelationship.

The valve mechanism 124 further includes an actuator 152, which includesa shaft 154 coupled to the valve 142 and extending through a lower bore158 formed at the bottom of the vial container 112. Unlike the actuator52 illustrated in FIGS. 2 and 3, the actuator 152 does not include aboss or any part that protrudes from the vial container, so that thebottom surface of the vial 110 is flush or recessed to facilitate thestorage of the vial 110 in an upright manner. Instead, the shaft 154 isconfigured, such that a tool can be used to externally manipulate thevalve mechanism 124 to selectively locate the valve 142 within thealiquot chamber 122 and displace the valve 142 from the aliquot chamber122, as will be described in further detail below. The annular flange146 of the valve 142 and the shaft 154 may be conveniently formed fromthe same material as a unibody design, e.g., in an injection moldingprocess using a plastic material, such as polypropylene.

As best shown in FIG. 11, in order to prevent fluid communicationbetween the aliquot chamber 122 and the external environment that mayotherwise occur through the lower bore 158, the valve mechanism 124includes an O-ring seal 160 seated within an annular recess 162 formedaround the circumference of the shaft 154. The O-ring seal 160 axiallymoves within an enlarged upper bore 164 between the lower bore 158 andthe aliquot chamber 122. In the illustrated embodiment, the upper bore164 is defined by an inner annular flange 166 formed within vialcontainer 112, so that the O-ring seal 160 bears against an innersurface 168 of the inner annular flange 166. Thus, the O-ring seal 160axially moves within the upper bore 164 in a sealing arrangement toallow the actuator shaft 154, and thus, the valve 142, to move up ordown relative to aliquot chamber 122, while preventing fluidcommunication between the aliquot chamber 122 and the externalenvironment through the lower bore 158.

In the embodiment illustrated in FIGS. 9 and 10, axial movement of theactuator shaft 154 is accomplished in the same manner as the actuatorshaft 54 described with respect to FIGS. 2 and 3. That is, the actuatorshaft 154 may be rotationally translated to operably move the valve 142relative to the aliquot chamber 122. To this end, a threaded arrangement170 is provided between the actuator shaft 154 and the upper bore 158.Thus, clockwise rotation of the shaft 154 causes the valve 142 to moveaxially upward, and counterclockwise rotation of the shaft 154 causesthe valve 142 to move axially downward. A slot or other suitable patternis formed at the end of the shaft 154 to allow a tool, such as a screwdriver, to be mated with the shaft 154 for subsequent rotation thereof.

Alternatively, in the same manner described above with respect to FIGS.5 and 6, the valve mechanism may be configured, such that the shaft 154need only be axially translated, i.e., without rotational translation.Or, in the same manner described above with respect to FIGS. 7 and 8,the valve mechanism may be configured, such that the valve seals thealiquot chamber 122 at the interface with the collection chamber 120,e.g., creating a seal between the upper-most edge of the inner annularflange 128 and a valve.

To allow user access to the aliquot sample, the vial container 112includes an access port 172 adjacent the aliquot chamber 122 and asealing mechanism in the form of septum 174 seated within the accessport 172 to seal it, thereby preventing fluid communication from thealiquot chamber 122 through the access port 172 until the user is readyto remove the aliquot sample from the aliquot chamber 122 forexamination. User access to the aliquot sample can be accomplished,e.g., by puncturing the septum 174 with a syringe and drawing thealiquot sample from the aliquot chamber 122 into the syringe.Alternatively, a seal may be bonded on top surface of the vial cap 114above the access port 172 or a screw-on plug or cap can be used to sealthe access port 172.

Having described the structure and function of several embodiments ofvials, a method of processing a vial will now be described withreference to FIG. 12. The illustrated method will be described in thecontext of triaging patients for precursors of cervical cancer.

First, the vial cap is removed from the vial container and a fluid-basedcervical-vaginal sample is placed within the collection chamber of thevial container (step 200). This step can typically be accomplished atthe physician's office. In the illustrated method, the cervical-vaginalsample taken as part of the routine Pap smear. In particular, cells arescraped from the cervix of the patient and mixed into a preservativesolution, such as PreservCyt® transport medium, contained within thecollection chamber of the vial container. Next, the vial cap is placedback on the vial container, and the vial with the collected fluid-basedsample, is transferred to a cytological laboratory (step 202).

At the cytological laboratory, the fluid-based sample is agitated todisburse the cells (step 204), and the aliquot chamber in the vial isunsealed from the collection chamber while the vial cap is mated withthe vial container (step 206). In the embodiments illustrated in FIGS. 2and 3, this is accomplished by rotating the boss 56 on the vial cap 14in the clockwise direction to displace the valve 42 out of the aliquotchamber 22. In the embodiment illustrated in FIGS. 5 and 6, this isaccomplished by pushing the boss 56 on the vial cap 84 downward againstthe urging force of the spring 88 to displace the valve 42 out of thealiquot chamber 22. In the embodiment illustrated in FIGS. 7 and 8, thisis accomplished by rotating the boss 56 on the vial cap 94 in theclockwise direction to displace the valve 98 away from the lower-mostedge 100 of the inner annular flange 28. In the embodiment illustratedin FIGS. 9 and 10, this is accomplished by rotating the shaft 154 in theclockwise direction with the special tool to displace the valve 142 outof the aliquot chamber 122.

Next, an aliquot of the sample is flowed from the collection chamberinto the unsealed aliquot chamber while the sample is isolated from anenvironment exterior to the vial (i.e., while the vial cap is mated withthe vial container) (step 208). In vials wherein the aliquot chamber isintegrated into the vial cap, such as the embodiments illustrated inFIGS. 2 and 3, FIGS. 5 and 6, and FIGS. 7 and 8, and FIGS. 9 and 10,this can be accomplished by flipping the vials upside down. In vialswherein the aliquot chamber is integrated into the vial container, suchas the embodiment illustrated in FIGS. 9 and 10, the aliquot of thesample will flow from the collection chamber into the aliquot chamber inresponse to unsealing the aliquot chamber from the collection chamber instep 204.

Alternatively, if screw-type valve mechanisms are used, such as thoseillustrated in FIGS. 2 and 3, FIGS. 7 and 8, and FIGS. 9 and 10, theagitation, unsealing, and flowing steps 204, 206, 208 can be performedat the physician's office prior to transferring the respective vial tothe cytological laboratory.

Next, the aliquot chamber is sealed from the collection chamber toisolate the aliquot chamber from the remaining portion of the samplecontained in the collection chamber (step 210). In the embodimentsillustrated in FIGS. 2 and 3, this is accomplished by rotating the boss56 on the vial cap 14 in the counterclockwise direction to relocate thevalve 42 into the aliquot chamber 22. In the embodiment illustrated inFIGS. 5 and 6, this is accomplished simply be relieving downwardpressure from the boss 56 on the vial cap 84, and allowing the urgingforce of the spring 88 to move the valve 42 back into the aliquotchamber 22. In the embodiment illustrated in FIGS. 7 and 8, this isaccomplished by rotating the boss 56 on the vial cap 94 in thecounterclockwise direction to abut the valve 98 against the lower-mostedge 100 of the inner annular flange 28. In the embodiment illustratedin FIGS. 9 and 10, this is accomplished by rotating the shaft 154 in thecounterclockwise direction with the special tool to relocate the valve142 into the aliquot chamber 122.

Next, the vial cap is unmated from the vial container to expose, andthereby provide access, to the remaining sample portion in thecollection chamber (step 212), and at least some of the remaining sampleportion is transferred from the collection chamber to a microscope slidewhile the aliquot chamber is sealed from the collection chamber (step214). Typically, exposing the collection chamber to the externalenvironment may expose the remaining sample portion to contaminants(e.g., HPV) at the molecular level. This may be especially true if theslide preparation process is performed by an automated specimenpreparation system where molecular contaminants are often found. Withouttaking additional precautions, such molecular contaminants can be foundin an aerosol or within filtered cell solution in the plumbing of theautomated specimen preparation system where it can be transferred fromvial to vial. However, because the aliquot sample in the aliquot chamberis isolated from the collection chamber, it will not be exposed to anycontaminants that may enter the collection chamber.

Next, the slide specimen is reserved for cytological screening of thesample for precursors of cervical cancers (step 216), and the samplealiquot is reserved for DNA testing, e.g., for the present of high-riskHPV in the sample (step 218). Next, the slide is cytological screened,e.g., for precursors of cervical cancers (step 220). This can beaccomplished in the same laboratory at which the slide was prepared, oralternatively, can be transferred to another laboratory. In the casewhere no abnormal cells are found, the patient is returned to a routinePap smear schedule (step 222). In the case of an ASC-US+ result, thepatient is scheduled for a colposcopy/biopsy at the physician's office(step 224). In the case of an ASC-US result, the aliquot sample isremoved from the aliquot chamber via the access port within the vial capor vial container (step 226), and a reflex DNA test is performed on thealiquot sample reserved in step 218 for the presence of high-risk HPV(step 228). This can be accomplished using Digene's Hybrid Capture IIHPV DNA assay. If the presence of high-risk HPV is detected in thesample, the patient is scheduled for a colposcopy/biopsy at thephysician's office (step 230), or alternatively may be placed on aschedule with increased Pap smear intervals. If the presence ofhigh-risk HPV is not detected in the sample, the patient may then bereturned to a routine Pap smear schedule (step 232). Optionally, otherDNA tests, e.g., to detect the presence of such as Chlamydia trachomatisand Neisseria gonorrhoeae, may be performed. These other DNA tests, oreven the HPV DNA test, can be alternatively performed in parallel withthe cytological screening of the slide.

Although particular embodiments of the present invention have been shownand described, it should be understood that the above discussion is notintended to limit the present invention to these embodiments. It will beobvious to those skilled in the art that various changes andmodifications may be made without departing from the spirit and scope ofthe present invention. Thus, the present invention is intended to coveralternatives, modifications, and equivalents that may fall within thespirit and scope of the present invention as defined by the claims.

1. A method of processing a vial having a collection chamber containinga fluid-based cervical-vaginal sample, a valve mechanism, and an aliquotchamber, wherein the valve mechanism comprises or is coupled to a wallof the aliquot chamber, and wherein the valve mechanism is biased toseal the aliquot chamber from the collection chamber unless activelyactuated, the method comprising: actuating the valve mechanism to unsealthe aliquot chamber from the collection chamber; flowing an aliquot ofthe sample from the collection chamber into the aliquot chamber whilethe collection chamber is isolated from an environment exterior to thevial; terminating active actuation of the valve mechanism, wherein thebiased valve mechanism automatically seals the aliquot chamber from thecollection chamber to isolate the aliquot sample from a remainingportion of the sample in the collection chamber; transferring at leastsome of the remaining sample portion from the collection chamber to amicroscope slide while the aliquot chamber is sealed from the collectionchamber; reserving the slide for cytological screening of the sample forprecursors to cervical cancer; and reserving the aliquot sample fordeoxynucleic acid (DNA) testing to determine the presence of high-riskHuman Papilloma Virus (HPV) in the sample.
 2. The method of claim 1,further comprising examining the slide to cytologically screen thesample for the cervical cancer precursors.
 3. The method of claim 1,further comprising DNA testing the aliquot sample to determine thepresence of high-risk HPV in the sample.
 4. The method of claim 3,wherein the DNA testing is performed in response to an abnormal resultof the cytological screening.
 5. The method of claim 4, wherein the DNAtesting is performed only in response to an Atypical Squamous Cell ofUndetermined Significance (ASC-US) result of the cytological screening.6. The method of claim 1, wherein the vial has a vial container carryingthe collection chamber and a vial cap mated with the vial container, themethod further comprising unmating the vial cap from the vial containerto expose the remaining sample portion within the collection chamber. 7.The method of claim 6, wherein the aliquot chamber is carried by thevial cap.
 8. A method of processing a vial having a collection chambercontaining a fluid-based sample, a valve mechanism, and an aliquotchamber, wherein the valve mechanism comprises or is coupled to the wallof the aliquot chamber, and wherein the valve mechanism is biased toseal the aliquot chamber from the collection chamber unless activelyactuated, the method comprising: actuating the valve mechanism to unsealthe aliquot chamber from the collection chamber; flowing an aliquot ofthe sample from the collection chamber into the aliquot chamber whilethe collection chamber is isolated from an environment exterior to thevial; terminating actuation of the valve mechanism, wherein the biasedvalve mechanism automatically seals the aliquot chamber from thecollection chamber to isolate the aliquot sample from a remainingportion of the sample in the collection chamber; removing at least someof the remaining sample portion from the collection chamber while thealiquot chamber is sealed from the collection chamber; wherein thefluid-based sample is a cervical-vaginal sample; and performing acytological examination on the removed remaining sample portion.
 9. Themethod of claim 8, wherein the at least some of the remaining sampleportion is transferred to a microscope slide.
 10. The method of claim 8,further comprising performing a deoxynucleic acid (DNA) test on thealiquot sample.
 11. The method of claim 10, wherein the DNA test isperformed in response to an abnormal result of the cytologicalexamination.
 12. The method of claim 8, wherein the vial has a vialcontainer carrying the collection chamber and a vial cap mated with thevial container, the method farther comprising unmating the vial cap fromthe vial container to expose the remaining sample portion within thecollection chamber.
 13. The method of claim 12, wherein aliquot chamberis carried by the vial cap.
 14. The method of claim 8, wherein the vialhas an access port, wherein the access port provides access between thealiquot chamber and the exterior of the vial, the method furthercomprising removing at least some of the aliquot portion from thealiquot chamber via the access port.